Viral DNA / RNA Ihe ntinye (Kọlụm)
A na-eji Kit Kit DNA mee ihe maka ịdị mfe, ngwa ngwa na ntụkwasị obi nke DNA na-ekesa n'efu (CFC-DNA) site na 1 mL ruo 4 mL plasma / serum. Usoro ọcha dị na chromatography nke kọlụm centrifugal ma jiri resin nke Sigma kewaa matriks. Enwere ike iji kit ahụ kewapụ CFC-DNA nke nha dịgasị iche site na ntanetị plasma / ọbara ọhụụ. Tụkwasị na nke a, enwere ike gbanwee olu elu site na 25 μl ruo 50 μl. Plasma / ọbara CFC-DNA ahụ dị ọcha ga-eleba anya na elu dị elu iji kwekọọ na ngwa ọ bụla dị ala, gụnyere PCR, PCR oge dị ukwuu, PCR methylation na-enyocha PCR na nyocha mkpocha Southern, microarray na NGS.
Gbasa DNA dị iche iche nwere ike ịdị iche na plasma na ọbara
Viral na nje nje DNA nwere ike iche
Kwesịrị ekwesị maka ụdị plasma na ọbara na-amalite nha ụdị (1 mL ~ 4 mL)
Enwere ike idozi olu elekere na mgbanwe nke 50 μL ~ 100 μL iji tinye uche na-ekesa DNA
Enwere ike ịtọpụ DNA na-ekesa n'efu na-enweghị ihe mgbochi
Enwere ike ime ka DNA dị elu dị na nkeji 40-45
Dakọtara na Tutu tubes nke DNA BCT nke Streck Cell na-adịghị ọcha
Ngwa
PCR
Ukwuu
Ndịda akara
PCR na-emetụ methylation
CpG n'usoro
Mgbochi mgbaze enzyme
Nchọpụta nje
Nchọpụta nje
microarray
NGS
